ACELL September 46/3
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چکیده
Kobuchi, Hirotsugu, Sashwati Roy, Chandan K. Sen, Hao G. Nguyen, and Lester Packer. Quercetin inhibits inducible ICAM-1 expression in human endothelial cells through the JNK pathway. Am. J. Physiol. 277 (Cell Physiol. 46): C403–C411, 1999.—The cell adhesion molecule intercellular adhesion molecule-1 (ICAM-1) plays a pivotal role in inflammatory responses. Quercetin (3,38,48,5,7-pentahydroxyflavone), a naturally occurring dietary flavonol, has potent anti-inflammatory properties. The effect of quercetin on ICAM-1 expression induced by agonists phorbol 12-myristate 13-acetate (PMA) and tumor necrosis factor-a (TNF-a) in human endothelial cell line ECV304 (ECV) was investigated. Quercetin treatment downregulated both PMAand TNF-ainduced surface expression, as well as the ICAM-1 mRNA levels, in ECV cells in a dose-dependent (10–50 μM) manner. Quercetin had no effect on PMAor TNF-a-induced nuclear factor-kB (NF-kB) activation. However, under similar conditions a remarkable dose-dependent downregulation of activator protein-1 (AP-1) activation was observed. This decrease in AP-1 activation was observed to be associated with the inhibitory effects of quercetin on the c-Jun NH2-terminal kinase (JNK) pathway. These results suggest that quercetin downregulates both PMAand TNF-a-induced ICAM-1 expression via inhibiting both AP-1 activation and the JNK pathway.
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ACELL September 46/3
Dodd, J. S., J. A. Raleigh, and T. S. Gross. Osteocyte hypoxia: a novel mechanotransduction pathway. Am. J. Physiol. 277 (Cell Physiol. 46): C598–C602, 1999.—Bone is a unique tissue in which to examine mechanotransduction due to its essential role in weight bearing. Within bone, the osteocyte is an ideal cellular mechanotransducer candidate. Because osteocytes reside distant from the blood supp...
متن کاملACELL September 46/3
Zeiske, Wolfgang, Ilse Smets, Marcel Ameloot, Paul Steels, and Willy Van Driessche. Intracellular pH shifts in cultured kidney (A6) cells: effects on apical Na1 transport. Am. J. Physiol. 277 (Cell Physiol. 46): C469–C479, 1999.—We report, for the epithelial Na1 channel (ENaC) in A6 cells, the modulation by cell pH (pHc) of the transepithelial Na1 current (INa), the current through the individu...
متن کاملACELL September 46/3
Shepard, Allan R., and James L. Rae. Electrically silent potassium channel subunits from human lens epithelium. Am. J. Physiol. 277 (Cell Physiol. 46): C412–C424, 1999.—We describe the cloning and characterization of the first human members, hKv9.1 and hKv9.3, of the electrically silent delayed-rectifying-like K1 channel subfamily. Their modulatory effects on the electrically active subfamily m...
متن کاملACELL September 46/3
Stevens, Randel J., Jeffery S. Weinert, and Nelson G. Publicover. Visualization of origins and propagation of excitation in canine gastric smooth muscle. Am. J. Physiol. 277 (Cell Physiol. 46): C448–C460, 1999.—The origin and spread of excitation were visualized with fluo 3 fluorescence in tissues isolated from canine gastric antrum. Sheets of circular muscle (5 3 6 mm) had at least 1 (30%) and...
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JOHN M. PARK,1 ROSALYN M. ADAM,1 CRAIG A. PETERS,1 PAUL D. GUTHRIE,1 ZIJIE SUN,2 MICHAEL KLAGSBRUN,3 AND MICHAEL R. FREEMAN3 1Urologic Laboratory, Department of Urology, 3Laboratory for Surgical Research, Children’s Hospital, and Department of Surgery, Harvard Medical School, Boston, Massachusetts 02115; and 2Departments of Surgery and Genetics, Stanford University School of Medicine, Stanford,...
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